Survey Design

The eDNA Consultancy brings expertise and innovation to provide clarity in DNA based monitoring

A minimalist flat illustration of a white fountain pen pointing at a folded paper map.
A minimalist flat illustration of a white fountain pen pointing at a folded paper map.

Well-designed projects turn powerful eDNA data into clear insights about biodiversity. We can combine your ecological knowledge with our eDNA expertise to build surveys that are robust, straightforward to implement and tailored to your target species and habitats. Proper survey design helps you to confidently collect samples and generate the evidence you need.

Book a survey design meeting

“eDNA surveys have incredible potential, the better the design, the clearer the story they tell about our environment.”

Why survey design matters

A good eDNA survey aligns ecological context with an array of molecular methods so your results reflect the patterns in species presence and distribution. Careful choices about timing, sample number, replication, and sample type can increase detection probability, reduce false negatives, and give you data you can use with confidence (a false negative occurs when a species is present but goes undetected, poor sampling strategy and technique increases the likelihood of this happening). It can also ensure efficient use of budget and streamline field research time.

Sampling design considerations

Time of year and species activity levels
DNA shedding rates of target species
e.g. reproductive timing
Target species distribution and local abundance
Simple black and white line art calendar icon with check marks showing completed daily tasks.
Simple black and white line art calendar icon with check marks showing completed daily tasks.
White line art illustration of laboratory microcentrifuge tubes inside a plastic bag on a black background.
White line art illustration of laboratory microcentrifuge tubes inside a plastic bag on a black background.
Habitat type and environmental conditions that may affect DNA recovery
Sampling method and preservation
(soil, water, air, or terrestrial)
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Sample specific variables
e.g. in aquatic surveys, dilution and flow dynamics
A black and white line drawing of a fish and survey points
A black and white line drawing of a fish and survey points
Simple black and white line drawing of a DNA double helix structure icon on a white background.
Simple black and white line drawing of a DNA double helix structure icon on a white background.
Black and white line art icon of cattails and marsh grass growing by the water.
Black and white line art icon of cattails and marsh grass growing by the water.
White outline illustration of laboratory centrifuge tubes inside a plastic bag on a black background.
White outline illustration of laboratory centrifuge tubes inside a plastic bag on a black background.
example sampling plan
example sampling plan
Advice on existing assays and primer selection
A defensible survey plan
Sampling framework

Sample number, maps optimal sample site locations, replication, and timing.

Sampling Design Output

aligned to meet your goals and detect your target species.

Custom primer design and validation in silico and in vitro if required

Efficient sampling strategy

that balances effort, cost, and detection probability.

Clean, reproducible methods and documentation

For projects requiring predictive or management applications, we can extend your sampling design to include:

Habitat suitability modelling

We map habitat suitability to identify the most likely sites for your target taxa, helping you focus survey effort where detection is most probable and reducing overall project costs.

Advice on which environmental variables to collect, such as water chemistry and habitat condition, to link species presence with habitat factors and strengthen interpretation of your eDNA results.

Following your survey, we can model true species presence by accounting for imperfect detection. These models guide future sampling, reveal key environmental drivers, and help identify false negatives.

Topographic heat map showing low, medium, and high intensity levels in shades of teal and cream.
Topographic heat map showing low, medium, and high intensity levels in shades of teal and cream.
a diagram of occupancy modelling
a diagram of occupancy modelling

Project design and eDNA data interpretation support

Abstract 3D topographical map illustration with teal layered terrain and black location pin icons.
Abstract 3D topographical map illustration with teal layered terrain and black location pin icons.
Species distribution modelling
Occupancy modelling
Metadata advice

We always recommend surveying a smaller area robustly rather than a larger area poorly. By combining eDNA detections with environmental and spatial variables, we can predict species occurrence across unsampled areas, expanding your survey’s geography.

These modelling approaches translate eDNA detections into ecologically meaningful insights, helping you prioritise habitats, refine monitoring strategies, and support defensible conservation decisions.

A light blue clipboard icon with a checkmark list, water drop, and plant sprout for environmental sustainability.
A light blue clipboard icon with a checkmark list, water drop, and plant sprout for environmental sustainability.
Metadata design
Map layer sourcing and preparation
Targeted literature review

Identify key environmental and ecological variables influencing your target taxa to guide model development and sampling strategy.

Project Design Output

Compile and process relevant spatial layers (habitat, elevation, hydrology, land cover, climate, etc.) ready for use in modelling.

Develop custom metadata collection frameworks (e.g. water chemistry, habitat metrics, land use, and spatial data) to ensure compatibility with modelling approaches and long-term monitoring.

Modelling with clear method documentation and reporting.

Link HSM, occupancy, and SDM outputs to create a unified understanding of habitat quality, species occurrence, and landscape connectivity.

Cross-model integration
Map of Ireland showing pie charts of sampling data from Lough Corrib, White Lough, Muckanagh, Derg, and Owel.
Map of Ireland showing pie charts of sampling data from Lough Corrib, White Lough, Muckanagh, Derg, and Owel.
A topographic heat map of Somerset, UK, displaying environmental data across Bristol, Taunton, and Exmoor.
A topographic heat map of Somerset, UK, displaying environmental data across Bristol, Taunton, and Exmoor.
Sampling Design

Cost savings are typically achieved for projects with more than 10 samples. For surveys with 10+ samples, we recommend a full sample design package, starting from £50 and capped at £500.

£5/sample
Project design
£250/half day

Quoted based on the time required, reflecting project complexity and the availability of relevant map layers or environmental data. A detailed quote will be provided once the scope and complexity of the work are fully understood.

Buffy and Laura smiling at each other
Buffy and Laura smiling at each other

Pricing

Book a survey design meeting

Client's aim

To determine where infrastructure improvements should be made to restore historic fish populations.

Our input

We modelled sample collection points based on locations of barriers to fish movement and designed a complementary water quality monitoring plan. Collected and analysed eDNA and water chemistry samples

The output

Full report with publication quality data analysis. Statistically robust analyses contextualising eDNA findings with additional ecological parameters, mapping and recommendations.

Example Consultancy project

A serene freshwater marsh with green reeds and algae floating on the water's surface.
A serene freshwater marsh with green reeds and algae floating on the water's surface.
Minimalist graphic illustration of a fountain pen nib pointing at a folded paper map icon.
Minimalist graphic illustration of a fountain pen nib pointing at a folded paper map icon.

Links to our other services

Other services
A diagram of a laboratory test tube containing environmental DNA samples from insects, air, soil, and water.
A diagram of a laboratory test tube containing environmental DNA samples from insects, air, soil, and water.
Illustration of scientific lab sample centrifuge tubes inside a transparent plastic bag.
Illustration of scientific lab sample centrifuge tubes inside a transparent plastic bag.
Training
Community science
Line art illustration of a laboratory pipette, gear, and DNA strand representing genetic engineering.
Line art illustration of a laboratory pipette, gear, and DNA strand representing genetic engineering.

The eDNA Consultancy

Where our expertise meets yours

ADDRESS

Unit 1, Tanhouse Farm, Frampton on Severn, Gloucestershire, GL2 7EH, GB

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